Mutations in the gene cdk2, which encodes a cyclin-dependent kinase, have been implicated in breast cancer. You have cloned out the cdk2 gene from affected members of one family with a history of breast cancer (sequence labelled cdk2-mut).

Compare the sequences of the wild type and mutant cdk2 genes. What is the nucleotide change in the mutant allele? What change does that cause in the amino acid sequence?

>cdk2  wild type
cttcatggagaacttccaaaaggtggaaaagatcggagagggcacgtacggagttgtgtacaaagccagaaacaagttga
cgggagaggtggtggcgcttaagaaaatccgcctggacactgagactgagggtgtgcccagtactgccatccgagagatc
tctctgcttaaggagcttaaccatcctaatattgtcaagctgctggatgtcattcacacagaaaataaactctacctggt
ttttgaatttctgcaccaagatctcaagaaattcatggatgcctctgctctcactggcattcctcttcccctcatcaaga
gctatctgttccagctgctccagggcctagctttctgccattctcatcgggtcctccaccgagaccttaaacctcagaat
ctgcttattaacacagagggggccatcaagctagcagactttggactagccagagcttttggagtccctgttcgtactta
cacccatgaggtggtgaccctgtggtaccgagctcctgaaatcctcctgggctgcaaatattattccacagctgtggaca
tctggagcctgggctgcatctttgctgagatggtgactcgccgggccctattccctggagattctgagattgaccagctc
ttccggatctttcggactctggggaccccagatgaggtggtgtggccaggagttacttctatgcctgattacaagccaag
tttccccaagtgggcccggcaagattttagtaaagttgtacctcccctggatgaagatggacggagcttgttatcgcaaa
tgctgcactacgaccctaacaagcggatttcggccaaggcagccctggctcaccctttcttccaggatgtgaccaagcca
gtaccccatcttcgactctgatagccttcttgaagcccccagccctaatctcaccctctcctccagtgtgggcttgacca
ggcttgcgcttgggctatttggactcaggtgggccctctgaacttgccttaaac

>cdk2 mutant
cttcatggagaacttccaaaaggtggaaaagatcggagagggcacgtacggagttgtgtacaaagccagaaacaagttga
cgggagaggtggtggcgcttaagaaaatccgcctggacactgagactgagggtgtgcccagtactgccatccgagagatc
tctctgcttaaggagcttaaccatcctaatattgtcaagctgctggatgtcattcacacagaaaataaactctacctggt
ttttgaatttctgcaccaagatctcaagaaattcatggatgcctctgctctcactggcattcctcttcccctcatcaaga
gctatctgttccagctgctccagggcctagctttctgccattctcatcgggtcctccaccgagaccttaaacctcagaat
ctgcttattaacacagagggggccatcaagctagcagactttggactagccagagcttttggagtccctgttcgtactta
cacccatgaggtggtgaccctgtggtaccgagctcctaaaatcctcctgggctgcaaatattattccacagctgtggaca
tctggagcctgggctgcatctttgctgagatggtgactcgccgggccctattccctggagattctgagattgaccagctc
ttccggatctttcggactctggggaccccagatgaggtggtgtggccaggagttacttctatgcctgattacaagccaag
tttccccaagtgggcccggcaagattttagtaaagttgtacctcccctggatgaagatggacggagcttgttatcgcaaa
tgctgcactacgaccctaacaagcggatttcggccaaggcagccctggctcaccctttcttccaggatgtgaccaagcca
gtaccccatcttcgactctgatagccttcttgaagcccccagccctaatctcaccctctcctccagtgtgggcttgacca
ggcttgcgcttgggctatttggactcaggtgggccctctgaacttgccttaaac

Back to courses